Mouse Protein Wnt-3a (WNT3A) ELISA Kit from MyBioSource.com

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Mouse Protein Wnt-3a (WNT3A) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Protein Wnt-3a (also known as WNT3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse WNT3A antibody. WNT3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse WNT3A Antibody is added and binds to WNT3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse WNT3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm